Question 1

What is a peptide reconstitution calculator?

Accepted Answer

A peptide reconstitution calculator computes exactly how much liquid to draw into a syringe based on your vial size, bacteriostatic water volume, and target dose. Because peptide doses are measured in micrograms, even a small measurement error matters. The calculator outputs a single IU number — the mark on your U-100 insulin syringe to draw to.

Question 2

What formula does this peptide calculator use?

Accepted Answer

Concentration (mcg/mL) = (vial mg × 1000) ÷ BAC water mL. Volume (mL) = dose mcg ÷ concentration. IU = volume × 100. Example: 5 mg vial in 2 mL → 2,500 mcg/mL. 250 mcg dose → 0.1 mL → 10 IU.

Question 3

How many IU do I draw for 250 mcg of BPC-157?

Accepted Answer

With a 5 mg vial in 2 mL BAC water (2,500 mcg/mL): 250 ÷ 2,500 = 0.1 mL = 10 IU. If you used 1 mL of BAC water (5,000 mcg/mL): 250 ÷ 5,000 = 0.05 mL = 5 IU. Always enter your actual BAC water volume.

Question 4

How do I calculate semaglutide dose in IU?

Accepted Answer

For 0.5 mg vial in 1 mL: concentration = 500 mcg/mL. A 0.25 mg (250 mcg) dose = 50 IU. Select 'Semaglutide' from the dropdown and the standard values auto-fill.

Question 5

What is a U-100 insulin syringe and why use it for peptides?

Accepted Answer

U-100 syringes are calibrated for insulin at 100 units per mL, so 1 IU = 0.01 mL exactly. This makes the math simple and the syringes accurate at the small volumes peptide research requires. They're available at any pharmacy, 29–31 gauge, in 0.3–1 mL sizes.

Question 6

What happens if my IU result is above 100?

Accepted Answer

You exceed your syringe capacity. Fix by: (1) switching to a larger syringe (change syringe size in the calculator), (2) reconstituting with less BAC water to increase concentration, or (3) splitting the dose into two injections.

Question 7

How much bacteriostatic water should I add?

Accepted Answer

1–2 mL is standard for most peptides. More water lowers concentration and increases injection volume; less water raises concentration and lowers volume. 2 mL is a good default for 5–10 mg vials — doses land in the 10–50 IU range, which is easy to measure accurately.

Question 8

What is the difference between mg and mcg in the calculator?

Accepted Answer

mg (milligrams) is what's on the vial — the total peptide amount. mcg (micrograms) is how doses are measured — 1 mg = 1,000 mcg. For GLP-1 agonists like semaglutide, toggle the unit switch to mg on the dose field.

Question 9

How many shots will I get from my vial?

Accepted Answer

Shots per vial = (vial mg × 1,000) ÷ dose mcg. A 5 mg vial with a 250 mcg dose gives 20 shots. At 2 shots/week, that's 70 days. The calculator shows both numbers in the results panel.

Question 10

Can I use this calculator for GLP-1 agonists?

Accepted Answer

Yes — semaglutide, tirzepatide, and all GLP-1 agonists use the same reconstitution math. Select from the dropdown or enter values manually. Use the mcg⇄mg toggle since GLP-1 doses are typically in mg.

Question 11

Is this peptide calculator accurate?

Accepted Answer

Yes, for reference purposes. The formula is standard reconstitution chemistry, verified by Dr. James T. Walker, PhD. Real-world accuracy depends on precise vial content and BAC water measurement.

Question 12

Where can I buy peptides verified by this calculator?

Accepted Answer

We rank 32+ research peptide suppliers by COA quality, HPLC/MS purity verification, shipping, and support. See our vendor rankings at /vendors — only COA-verified suppliers are listed.

Vial	+ 2 mL	+ 5 mL	+ 10 mL
2 mg	100 mcg / 1 IU	40 mcg / 1 IU	20 mcg / 1 IU
5 mg	250 mcg / 1 IU	100 mcg / 1 IU	50 mcg / 1 IU
10 mg	500 mcg / 1 IU	200 mcg / 1 IU	100 mcg / 1 IU

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Reconstitution Reference

Peptide	Vial	BAC Water	Dose	IU to Draw
BPC-157	5 mg	2 mL	250 mcg	10 IU
BPC-157	5 mg	2 mL	500 mcg	20 IU
Semaglutide	0.5 mg	1 mL	0.25 mg	50 IU
Semaglutide	2 mg	2 mL	0.5 mg	50 IU
Tirzepatide	5 mg	1 mL	2.5 mg	50 IU
Tirzepatide	10 mg	2 mL	5 mg	100 IU
TB-500	10 mg	2 mL	5 mg	10 IU
CJC-1295	2 mg	2 mL	100 mcg	10 IU
Ipamorelin	2 mg	2 mL	200 mcg	20 IU
PT-141	10 mg	2 mL	1 mg	20 IU
Sermorelin	3 mg	3 mL	300 mcg	30 IU
AOD-9604	5 mg	2 mL	300 mcg	12 IU