Verified by Dr. James T. Walker, PhD — Biochemist & Research DirectorBased on published research dosing protocols
Blend StackBeginner1–3 injections daily

CJC-1295 + Ipamorelin Calculator

The most studied GH peptide combination — activating two separate pituitary pathways simultaneously for synergistic growth hormone pulses 5–10× larger than either peptide alone.

CJC-1295Ipamorelin

Syringe Size

Which syringe will you use? (applies to all peptides below)

CJC-1295

GHRH receptor activation (accelerator)

Full guide

Vial Size

BAC Water

Dose per Injection

Concentration

1.0 mg/mL

Volume

0.100 mL

Draw to

10.0 IU

Doses / vial

20

Ipamorelin

GHSR activation + somatostatin inhibition

Full guide

Vial Size

BAC Water

Dose per Injection

Concentration

2.5 mg/mL

Volume

0.080 mL

Draw to

8.0 IU

Doses / vial

25

Timing: Before sleep (essential) + optionally fasted morning. The step-by-step guide below covers exactly when and how to inject each peptide.

Stack Overview

Peptides2 compounds
Frequency1–3 injections daily
TimingBefore sleep (essential) + optionally fasted morning
GoalGrowth Hormone Optimization
DifficultyBeginner

What You'll Need

Go through this list before your first session. Check each item as you gather it.

Step-by-Step Guide

Follow these steps in order. Each step assumes you have no prior experience. Read each one fully before doing it.

This combination is most effective when injected on an empty stomach — ideally 2+ hours after your last meal and at least 30 minutes before eating again. The most important injection of the day is the one you take 30–60 minutes before sleep, because growth hormone is naturally secreted most during the first few hours of deep sleep. Eating carbohydrates or fats raises insulin, which directly suppresses the GH response. Protein alone has less impact. The bottom line: eat your last meal 2+ hours before your injection.

Tip: If you're doing just one injection per day, make it the pre-sleep injection. The timing matters more than the number of injections.

Wash your hands thoroughly with soap and water for 20 seconds. Dry with a clean paper towel. Lay a fresh paper towel on your workspace. Place both peptide vials and the bacteriostatic water vial, your syringes, and alcohol swabs on the surface.

Using a fresh alcohol swab for each, wipe the rubber tops of the CJC-1295 vial, the Ipamorelin vial, and the bacteriostatic water vial. Wipe in one direction — don't scrub back and forth. Let each top air-dry for 10–15 seconds. The alcohol needs to evaporate fully before you insert a needle.

Alcohol that has not fully evaporated can be drawn into the syringe and potentially degrade the peptide.

Draw the calculated amount of bacteriostatic water into a syringe. Insert the needle into the CJC-1295 vial and slowly drip the water down the inside wall of the glass — not directly onto the powder. This should take 10–15 seconds. Remove the needle, set the vial down, and wait 60 seconds. Then pick it up and gently swirl (do not shake) in slow circles until the powder is completely gone. The solution will be clear and colorless.

Tip: CJC-1295 dissolves quickly. If you still see powder after 90 seconds of gentle swirling, wait another 60 seconds — the powder will dissolve on its own. Never shake.

Repeat the exact same process for the Ipamorelin vial — draw the correct amount of bacteriostatic water, inject it slowly down the glass wall, wait 60 seconds, then gently swirl until fully dissolved. Label both vials with today's date and the peptide name.

Invert the CJC-1295 vial. Insert a fresh insulin syringe needle through the rubber top. Pull the plunger back slowly to draw your calculated dose (check the IU mark from the calculator above). Slightly overshoot, then slowly push back to your exact target mark to push out any bubbles and ensure a precise dose.

Tip: If you see a small air bubble, hold the syringe with the needle pointing up, flick the barrel gently, and slowly push the bubble out.

The most common approach is to draw both peptides into the same syringe immediately before injecting — this saves a needle stick. After drawing CJC-1295, keep the same syringe, insert it into the Ipamorelin vial, and draw your Ipamorelin dose. The combined volume should still fit within your syringe. Alternatively, use two separate syringes and inject each at a different site — both are valid approaches. Never pre-mix in a syringe and store — only combine immediately before injection.

The most practical sites for subcutaneous injection are the abdomen (pinch an inch of skin below and to the side of your navel, 2+ inches away from the navel itself) or the upper outer thigh. Rotate sites between injections — don't inject the exact same spot every day. Pinch the skin, insert the needle at a 45° angle, and push the plunger slowly and steadily over 10 seconds.

Do not inject into muscle for this protocol — subcutaneous (under the skin, into the fat layer) is the correct route for both peptides.

Withdraw the needle smoothly. Apply gentle pressure with a dry cotton ball or gauze — do not rub, as this can increase bruising. Place the used syringe directly into your sharps container. Refrigerate both vials at 2–8°C.

Tip: Wait at least 30 minutes before eating after your injection to maintain the GH response window.

Dosing Schedule

Typical protocol (once daily, pre-sleep):

CJC-1295: 100–200 mcg subcutaneously, 30–60 minutes before sleep

Ipamorelin: 200–300 mcg subcutaneously at the same time (can be in the same syringe)

Frequency: Daily, 5–7 days per week

Enhanced protocol (twice daily):

Morning injection (fasted, 30+ min before breakfast): CJC-1295 100 mcg + Ipamorelin 200 mcg

Pre-sleep injection: CJC-1295 100–200 mcg + Ipamorelin 200–300 mcg

**Cycle length:** 8–12 weeks on, 4 weeks off is a common research cycle structure. Some protocols run continuously for 6 months with a 1-month break.

Why These Peptides Are Combined

CJC-1295 and Ipamorelin activate two completely different receptor systems in the pituitary gland, and combining them exploits a fundamental piece of physiology: the pituitary has two main "go" signals for releasing growth hormone, and hitting both at the same time is far more effective than hitting either one alone.

CJC-1295 (without DAC, also called Mod GRF 1-29) mimics growth hormone-releasing hormone (GHRH). It binds to the GHRH receptor on pituitary somatotroph cells and triggers GH synthesis and release. Think of it as the "accelerator" signal.

Ipamorelin mimics ghrelin and binds to the GHSR-1a receptor (the ghrelin receptor) on the same cells. It amplifies the GH release signal and — critically — also inhibits somatostatin, the "brake" hormone that normally limits GH secretion. By removing the brake while pressing the accelerator, the combination produces GH pulses that are substantially larger than either peptide can achieve independently.

The other key reason this combination is favored in research: ipamorelin is the most selective GHRP available. Unlike older compounds (GHRP-2, GHRP-6, hexarelin), ipamorelin does not significantly increase cortisol or prolactin, making it a cleaner research tool.

Frequently Asked Questions

Research Use Only. These calculators and protocols are reference tools for laboratory research purposes. They do not constitute medical advice. Always verify calculations independently. All compounds referenced are research chemicals intended for laboratory investigation only. Consult a qualified professional for any medical application.

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