Sermorelin + Ipamorelin Calculator
A nightly pre-sleep stack combining a GHRH analog and a selective GHRP to amplify the body's natural nocturnal growth hormone surge.
Syringe Size
Which syringe will you use? (applies to all peptides below)
Sermorelin
GHRH receptor activation before sleep
Vial Size
BAC Water
Dose per Injection
Concentration
2.0 mg/mL
Volume
0.100 mL
Draw to
10.0 IU
Doses / vial
30
Ipamorelin
Ghrelin receptor activation + somatostatin inhibition
Vial Size
BAC Water
Dose per Injection
Concentration
2.5 mg/mL
Volume
0.080 mL
Draw to
8.0 IU
Doses / vial
25
Stack Overview
What You'll Need
Step-by-Step Guide
Follow these steps in order. Each step assumes you have no prior experience. Read each one fully before doing it.
This injection must happen 30–60 minutes before you go to sleep, and at least 2 hours after your last meal. Growth hormone is suppressed by insulin, so food — especially carbohydrates — within 2 hours of the injection will blunt the GH response. Eating a large dinner at 7 PM and injecting at 9:30 PM before bed at 10 PM is a practical example. Protein alone has less impact on insulin, so if hunger is an issue, a small amount of lean protein is preferable to carbohydrates.
Wash your hands. Swab the top of the sermorelin vial and the bacteriostatic water vial. Let dry for 15 seconds. Draw your calculated amount of bacteriostatic water. Inject it slowly down the glass wall of the sermorelin vial — 10–15 seconds. Set it down, wait 60 seconds, then gently swirl until dissolved. The solution will be clear and colorless. Label the vial with the date. A 6 mg vial reconstituted with 3 mL gives 2,000 mcg/mL — at 200 mcg per dose, that is 30 doses per vial.
Same process: swab the vial top, slowly inject bacteriostatic water down the glass wall, wait 60 seconds, gently swirl. Label with the date. A 5 mg vial with 2 mL gives 2,500 mcg/mL — at 200 mcg per dose that is 25 doses per vial.
Using a fresh insulin syringe, draw your sermorelin dose first (check the IU mark from the calculator above). Then — without changing the syringe — draw your ipamorelin dose from the ipamorelin vial. The two peptides can be combined in the same syringe immediately before injection. The combined volume should be small (typically 0.10–0.30 mL total), so a 1 mL syringe has plenty of capacity.
Choose an injection site — the abdomen (1–2 inches from the navel) or upper thigh. Pinch about 1 inch of skin between your thumb and forefinger. Insert the needle at a 45° angle. Slowly push the plunger over 10 seconds. Release the skin, withdraw the needle, apply light pressure with a dry cotton ball. Dispose of the syringe in your sharps container.
The peptides will begin stimulating GH release within 15–30 minutes. Aim to be asleep within 60 minutes of the injection to synchronize the peptide-induced GH pulse with the natural nocturnal GH surge during deep sleep. Avoid screens, bright lights, and stress in this window — sleep quality directly affects the magnitude of the GH pulse regardless of peptide use.
Dosing Schedule
Daily (before sleep):
Sermorelin: 100–300 mcg subcutaneously, 30–60 minutes before sleep
Ipamorelin: 200–300 mcg subcutaneously at the same time (same syringe)
On an empty stomach — minimum 2 hours after last meal.
**Cycle structure:** 3–6 months on, 1 month off is a common research protocol. Some researchers run continuously for up to 6 months given sermorelin's historical pharmaceutical use and established safety profile.
Why These Peptides Are Combined
Sermorelin and ipamorelin work through the same two receptor systems as CJC-1295 and ipamorelin (GHRH receptor and ghrelin receptor respectively), but with shorter pharmacokinetics. Sermorelin has a half-life of approximately 10–20 minutes — brief enough to produce a sharp GH pulse rather than sustained GH elevation, closely mimicking the natural nocturnal GH secretory pattern.
The body's largest natural GH pulse occurs during the first few hours of deep (slow-wave) sleep. By administering sermorelin + ipamorelin 30–60 minutes before sleep, the peptide-induced GH pulse synchronizes with and amplifies this natural event. This timing strategy is considered advantageous in anti-aging and recovery research because it preserves the natural pulsatile GH rhythm rather than artificially elevating GH throughout the day.
The advantage of sermorelin over CJC-1295 without DAC in this specific protocol is the legacy pharmaceutical data: sermorelin was FDA-approved as Geref and has more clinical safety data than CJC-1295. For researchers prioritizing the pre-sleep timing specifically, the pharmacokinetics of both GHRH analogs are similar enough that either works well in this protocol.