BPC-157 + TB-500 Calculator

Wash your hands thoroughly with soap and water for at least 20 seconds, including between your fingers and up to your wrists. Dry with a clean paper towel. Lay a fresh paper towel on a flat, clean surface and place all your equipment on top of it. Good lighting is important — you need to see the liquid levels in your syringe clearly.

Using a fresh alcohol swab, wipe the rubber septum (the rubber top) of the BPC-157 vial in one direction — don't rub back and forth. Do the same with the TB-500 vial and the bacteriostatic water vial. Let all three vials sit for 10–15 seconds so the alcohol evaporates completely. Wet alcohol on the rubber can contaminate your solution.

Using your larger syringe (or a fresh insulin syringe), draw the calculated amount of bacteriostatic water. Insert the needle into the BPC-157 vial at an angle and push it through the septum until just the tip enters. Slowly push the plunger so the water runs down the inside glass wall of the vial — never squirt it directly onto the powder. This takes 10–15 seconds. Remove the needle. Set the vial down gently. Do not shake it. After 60 seconds, pick it up and gently swirl it in slow circles until the powder has completely dissolved. The solution should be clear and colorless.

Repeat the same process for the TB-500 vial. Draw the calculated amount of bacteriostatic water for TB-500, inject it slowly down the glass wall of the vial, and let it rest for 60 seconds before gently swirling. TB-500 may take slightly longer to dissolve than BPC-157 — 2–3 minutes of gentle swirling is normal. The finished solution should be clear and colorless.

Using a permanent marker, write the reconstitution date on each vial and the peptide name if not already printed. This is important because both vials can look identical once reconstituted. Reconstituted peptides are good for 28 days when refrigerated — the date lets you track this.

Take a fresh insulin syringe. Wipe the BPC-157 vial top again with an alcohol swab and let it dry for a few seconds. Invert the vial (turn it upside down) and insert the needle through the septum. Pull the plunger back slowly to draw the correct volume — confirm against the IU mark shown in the calculator above. Pull the plunger a tiny bit past your target, then push back to the exact mark to ensure an accurate dose and remove any bubbles.

For BPC-157, the most common approach is subcutaneous injection in the abdomen (pinch about 1 inch of skin between your thumb and forefinger, insert the needle at a 45° angle, and slowly push the plunger over 5–10 seconds). Some researchers inject close to the site of injury (perilesional) for musculoskeletal targets. Release the skin, withdraw the needle, and apply gentle pressure with a clean cotton ball or gauze. Do not rub — just apply light pressure.

TB-500 is typically injected twice per week during a loading phase (e.g. Monday and Thursday), then once per week during maintenance. Draw your TB-500 dose from the reconstituted vial using a fresh syringe. The injection site and technique are the same as BPC-157 — subcutaneous, abdomen or thigh. Because TB-500 doses are larger in volume, the injection will take slightly longer. Keep the needle still and inject slowly over 10–15 seconds.

Place all used needles and syringes immediately into your sharps container. Never recap a used needle. Store both reconstituted vials in the refrigerator (2–8°C / 36–46°F), standing upright or lying on their side. Keep them away from the freezer compartment and away from direct light. They are stable for 28 days.