Sermorelin Dosage Guide: Reconstitution, Dose Calculator, and Injection Protocol
Complete guide to sermorelin vial reconstitution, injection volume tables, standard research dosage ranges, and timing protocols for GH-axis stimulation research.
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Sermorelin (GHRH 1-29) is a 29-amino acid GHRH analogue that stimulates pituitary GH release. It is widely used in growth hormone axis research. This guide covers reconstitution, injection volume calculation, and standard research dosing protocols.
What Vials Typically Come In
Sermorelin research vials are available in:
- 2 mg vials (most common)
- 5 mg vials
- 9 mg vials (for extended protocols)
- 15 mg vials (bulk research)
How to Reconstitute Sermorelin
Sermorelin is reconstituted with bacteriostatic water. It is relatively fragile — avoid excessive agitation.
Standard reconstitution for a 2 mg vial:
1. Swab the vial rubber stopper with alcohol; let dry
2. Draw 1–2 mL bacteriostatic water
3. Inject water gently down the inside wall of the vial
4. Swirl very gently — do not shake
5. Allow 2–3 minutes for full dissolution
Concentration examples:
- 2 mg + 1 mL BW → 2000 mcg/mL
- 2 mg + 2 mL BW → 1000 mcg/mL
- 5 mg + 2 mL BW → 2500 mcg/mL
The 1000 mcg/mL (2 mg in 2 mL) dilution is convenient for standard 200–300 mcg doses.
Standard Research Dosage Ranges
Published research uses:
| Protocol | Dose | Frequency |
|---|---|---|
| Standard GH stimulation | 200–300 mcg | Once daily (bedtime) |
| Enhanced GH stimulation | 200–300 mcg | Twice daily |
| Diagnostic stimulation test | 1 mcg/kg IV | Single dose |
Most referenced human protocol: 200–300 mcg subcutaneous, once daily at bedtime (to coincide with natural GH peak).
Injection Volume Table
Using a 1000 mcg/mL solution (2 mg in 2 mL BW):
| Target Dose | Volume to Inject |
|---|---|
| 100 mcg | 0.10 mL (10 units) |
| 150 mcg | 0.15 mL (15 units) |
| 200 mcg | 0.20 mL (20 units) |
| 250 mcg | 0.25 mL (25 units) |
| 300 mcg | 0.30 mL (30 units) |
Use the Dose Calculator to convert doses for any vial size or concentration.
Frequency, Timing, and Cycle Length
- Optimal timing: Bedtime injection aligns with the natural nocturnal GH pulse, amplifying GH secretion during slow-wave sleep
- Frequency: Once daily is standard; twice daily (morning + bedtime) can increase IGF-1 response
- Cycle length: Most studies run 3–6 months; some extend to 12 months
- Administration: Subcutaneous injection to abdomen or thigh is standard
Important Notes
- Sermorelin has a short half-life (~10–20 minutes); administer promptly after drawing into syringe
- Best administered fasted or 90 minutes post-meal for optimal GH response (avoid high-carbohydrate meals close to injection)
- Avoid combining with high somatostatin states (stress, high cortisol) for best results
Storage
- Lyophilised powder: -20°C for long-term; 2–8°C for short-term
- After reconstitution: 2–8°C; use within 30 days
- Protect from heat and light
Calculate Your Exact Injection Volume
Use our free dose calculator — enter any vial size and target dose to get the precise volume to draw up.
About the Author
KnowYourPeptide Research Team
KnowYourPeptide Research Team
Content produced by the KnowYourPeptide research and editorial team. All articles are written from peer-reviewed primary literature and reviewed for scientific accuracy by credentialed researchers and a board-certified physician before publication.
Meet the full editorial teamMedically Reviewed by Dr. Amanda Reid, MD
This article has been reviewed by Dr. Amanda Reid, MD (Board-Certified Internal Medicine), Know Your Peptide Medical Advisor, for scientific accuracy, safety information, and appropriate clinical context. Learn about our review process.