Epithalon Dosage Guide: Reconstitution, Injection Volumes, and Anti-Aging Protocol
Complete guide to Epithalon vial reconstitution, injection volume tables, standard research dosing for telomere lengthening and anti-aging research, and cycle protocol guidance.
Ready to calculate your exact injection volume?
Enter your vial size, concentration, and target dose to get precise volumes instantly.
Epithalon (Epitalon) is a tetrapeptide (Ala-Glu-Asp-Gly) studied for telomerase activation, telomere extension, and anti-aging effects. It was developed by Vladimir Khavinson at the St. Petersburg Institute of Bioregulation. This guide covers practical reconstitution and dosing for research use.
What Vials Typically Come In
Epithalon research vials are commonly available as:
- 5 mg vials (most standard)
- 10 mg vials
- 100 mg bulk (for extended protocols)
How to Reconstitute Epithalon
Standard reconstitution for a 5 mg vial:
1. Clean rubber stopper with alcohol swab; allow to dry
2. Draw 1–2 mL bacteriostatic water into a syringe
3. Inject water slowly down the inside vial wall
4. Swirl gently — do not shake or vortex
5. Allow 2 minutes for dissolution; solution should be clear
Concentration examples:
- 5 mg + 1 mL BW → 5000 mcg/mL
- 5 mg + 2 mL BW → 2500 mcg/mL
- 10 mg + 2 mL BW → 5000 mcg/mL
Standard Research Dosage Ranges
| Protocol | Dose | Frequency |
|---|---|---|
| Standard short course | 5–10 mg/day | Daily for 10–20 days |
| Extended course | 5 mg/day | 20 days, twice per year |
| Intensive protocol | 10 mg/day | 10 days, 2–3x per year |
| Maintenance | 5 mg/day | 10 days, once per year |
The most commonly referenced research protocol is 5 mg/day subcutaneous for 20 consecutive days, repeated 1–2 times per year.
Injection Volume Table
Using a 5000 mcg/mL solution (5 mg in 1 mL BW):
| Target Dose | Volume to Inject |
|---|---|
| 3 mg (3000 mcg) | 0.60 mL (60 units) |
| 5 mg (5000 mcg) | 1.00 mL (100 units) |
| 10 mg (10,000 mcg) | 2.00 mL — split into 2 sites |
Using a 2500 mcg/mL solution (5 mg in 2 mL BW):
| Target Dose | Volume to Inject |
|---|---|
| 2.5 mg | 1.00 mL (100 units) |
| 5 mg | 2.00 mL — split into 2 sites |
Use the Dose Calculator to instantly compute volumes for any vial size.
Frequency, Timing, and Cycle Length
- Timing: Morning subcutaneous injection is most common
- Duration: 10–20 consecutive daily injections per course
- Cycling: Most protocols use 2 courses per year; some research uses 3 courses annually
- Route: Subcutaneous injection to abdomen or thigh; intramuscular also reported in some studies
Storage
- Lyophilised vials: -20°C for long-term; 2–8°C for up to 3 months
- After reconstitution: 2–8°C; use within 28 days
Calculate Your Exact Injection Volume
Use our free dose calculator — enter any vial size and target dose to get the precise volume to draw up.
About the Author
KnowYourPeptide Research Team
KnowYourPeptide Research Team
Content produced by the KnowYourPeptide research and editorial team. All articles are written from peer-reviewed primary literature and reviewed for scientific accuracy by credentialed researchers and a board-certified physician before publication.
Meet the full editorial teamMedically Reviewed by Dr. Amanda Reid, MD
This article has been reviewed by Dr. Amanda Reid, MD (Board-Certified Internal Medicine), Know Your Peptide Medical Advisor, for scientific accuracy, safety information, and appropriate clinical context. Learn about our review process.