TB-500 Dosage Guide: Vial Reconstitution, Dosing Tables, and Research Protocol
Practical guide to TB-500 vial reconstitution, injection volume tables, standard research dose ranges, and administration frequency for systemic tissue repair protocols.
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TB-500 (Thymosin Beta-4 fragment) is a systemic tissue repair and cell migration peptide with documented effects across tendon, muscle, cardiac, and neural tissue. This guide covers practical reconstitution and dosing for research use.
What Vials Typically Come In
TB-500 is most commonly available for research in:
- 5 mg vials (most standard)
- 10 mg vials (for loading phase protocols)
- 2 mg vials (less common)
How to Reconstitute TB-500
TB-500 is a water-soluble peptide that reconstitutes easily in bacteriostatic water.
Standard reconstitution for a 5 mg vial:
1. Wipe the vial top with an alcohol swab; allow to dry
2. Draw 1–2 mL bacteriostatic water into a syringe
3. Inject water slowly along the vial wall — avoid direct contact with powder initially
4. Swirl gently (do not shake)
5. Wait 2–3 minutes; swirl again until fully dissolved
6. Clear, colourless solution is expected
Concentration examples:
- 5 mg + 1 mL BW → 5000 mcg/mL
- 5 mg + 2 mL BW → 2500 mcg/mL
- 5 mg + 5 mL BW → 1000 mcg/mL
Standard Research Dosage Ranges
TB-500 uses a loading phase followed by a maintenance phase, which is a notable difference from daily-dosed peptides like BPC-157.
| Phase | Dose | Frequency |
|---|---|---|
| Loading (weeks 1–6) | 4–10 mg | 2x per week |
| Maintenance (weeks 7–12+) | 2–5 mg | 1–2x per week |
Most research extrapolations for human use reference:
- Loading dose: 5–10 mg per week (split across 2 injections)
- Maintenance dose: 2.5–5 mg per week
Injection Volume Table
Using a 5000 mcg/mL reconstituted solution (5 mg in 1 mL BW):
| Target Dose | Volume to Inject |
|---|---|
| 2 mg (2000 mcg) | 0.40 mL (40 units) |
| 2.5 mg (2500 mcg) | 0.50 mL (50 units) |
| 5 mg (5000 mcg) | 1.00 mL (100 units) |
| 7.5 mg (7500 mcg) | 1.50 mL (two injections) |
For doses above 1 mL, split into two injection sites to improve absorption and reduce discomfort.
Calculate your exact injection volumes with the Dose Calculator.
Frequency, Timing, and Cycle Length
- Frequency: Twice weekly during loading; once or twice weekly during maintenance
- Injection route: Subcutaneous injection is standard; systemic distribution means site proximity to injury matters less than with some other peptides
- Timing: No specific time of day requirement
- Cycle length: 12 weeks loading + maintenance is the most referenced protocol; some researchers extend maintenance phase indefinitely
Storage
- Lyophilised vials: -20°C for long-term; 2–8°C for up to 6 months
- Reconstituted solution: 2–8°C; use within 28 days
- Avoid repeated freeze-thaw cycles post-reconstitution
Calculate Your Exact Injection Volume
Use our free dose calculator — enter any vial size and target dose to get the precise volume to draw up.
About the Author
KnowYourPeptide Research Team
KnowYourPeptide Research Team
Content produced by the KnowYourPeptide research and editorial team. All articles are written from peer-reviewed primary literature and reviewed for scientific accuracy by credentialed researchers and a board-certified physician before publication.
Meet the full editorial teamMedically Reviewed by Dr. Amanda Reid, MD
This article has been reviewed by Dr. Amanda Reid, MD (Board-Certified Internal Medicine), Know Your Peptide Medical Advisor, for scientific accuracy, safety information, and appropriate clinical context. Learn about our review process.