AOD-9604 Dosage Guide: Reconstitution, Injection Volumes, and Fat Loss Protocol
Practical guide to AOD-9604 vial reconstitution, injection volume calculation, standard research dosing for lipolysis and fat loss research, and fasting protocol guidance.
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AOD-9604 (Anti-Obesity Drug 9604) is a modified GH fragment (176-191) studied for its lipolytic properties and fat metabolism effects. Unlike GH itself, AOD-9604 does not significantly affect blood glucose or IGF-1. This guide covers practical reconstitution and dosing.
What Vials Typically Come In
AOD-9604 research vials are commonly available as:
- 5 mg vials (most standard)
- 2 mg vials
- 10 mg vials
How to Reconstitute AOD-9604
Standard reconstitution for a 5 mg vial:
1. Swab the stopper with alcohol; allow to dry
2. Draw 1–2 mL bacteriostatic water
3. Inject water slowly down the vial wall
4. Swirl gently — do not shake
5. Allow 2 minutes for complete dissolution
Concentration examples:
- 5 mg + 1 mL BW → 5000 mcg/mL
- 5 mg + 2 mL BW → 2500 mcg/mL
- 2 mg + 1 mL BW → 2000 mcg/mL
Standard Research Dosage Ranges
| Protocol | Dose | Frequency |
|---|---|---|
| Standard fat loss research | 300 mcg/day | Daily, fasted |
| Enhanced protocol | 500 mcg/day | Daily, fasted |
| Higher dose trials | 1000 mcg/day | Divided doses |
The most commonly referenced dose from clinical research is 300–500 mcg/day, administered subcutaneously in a fasted state.
Injection Volume Table
Using a 5000 mcg/mL solution (5 mg in 1 mL BW):
| Target Dose | Volume to Inject |
|---|---|
| 200 mcg | 0.04 mL (4 units) |
| 300 mcg | 0.06 mL (6 units) |
| 500 mcg | 0.10 mL (10 units) |
| 1000 mcg | 0.20 mL (20 units) |
Using a 2500 mcg/mL solution (5 mg in 2 mL BW):
| Target Dose | Volume to Inject |
|---|---|
| 300 mcg | 0.12 mL (12 units) |
| 500 mcg | 0.20 mL (20 units) |
| 1000 mcg | 0.40 mL (40 units) |
Use the Dose Calculator to calculate exact volumes for any concentration.
Frequency, Timing, and Cycle Length
- Timing: Fasted state is critical — administer 30–60 minutes before first meal (morning injection on an empty stomach)
- Frequency: Once daily
- Cycle length: 8–12 weeks; some research extends to 20 weeks
- Injection site: Subcutaneous to abdomen is most common; closer to fat deposits that are targeted
Storage
- Lyophilised vials: -20°C for long-term; 2–8°C short-term
- After reconstitution: 2–8°C; use within 28 days
Calculate Your Exact Injection Volume
Use our free dose calculator — enter any vial size and target dose to get the precise volume to draw up.
About the Author
KnowYourPeptide Research Team
KnowYourPeptide Research Team
Content produced by the KnowYourPeptide research and editorial team. All articles are written from peer-reviewed primary literature and reviewed for scientific accuracy by credentialed researchers and a board-certified physician before publication.
Meet the full editorial teamMedically Reviewed by Dr. Amanda Reid, MD
This article has been reviewed by Dr. Amanda Reid, MD (Board-Certified Internal Medicine), Know Your Peptide Medical Advisor, for scientific accuracy, safety information, and appropriate clinical context. Learn about our review process.