Dosage Guide 6 min read

AOD-9604 Dosage Guide: Reconstitution, Injection Volumes, and Fat Loss Protocol

Practical guide to AOD-9604 vial reconstitution, injection volume calculation, standard research dosing for lipolysis and fat loss research, and fasting protocol guidance.

By KnowYourPeptide Research Team
Doctor Reviewed
April 10, 2026

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AOD-9604 (Anti-Obesity Drug 9604) is a modified GH fragment (176-191) studied for its lipolytic properties and fat metabolism effects. Unlike GH itself, AOD-9604 does not significantly affect blood glucose or IGF-1. This guide covers practical reconstitution and dosing.

What Vials Typically Come In

AOD-9604 research vials are commonly available as:

  • 5 mg vials (most standard)
  • 2 mg vials
  • 10 mg vials

How to Reconstitute AOD-9604

Standard reconstitution for a 5 mg vial:

1. Swab the stopper with alcohol; allow to dry

2. Draw 1–2 mL bacteriostatic water

3. Inject water slowly down the vial wall

4. Swirl gently — do not shake

5. Allow 2 minutes for complete dissolution

Concentration examples:

  • 5 mg + 1 mL BW → 5000 mcg/mL
  • 5 mg + 2 mL BW → 2500 mcg/mL
  • 2 mg + 1 mL BW → 2000 mcg/mL

Standard Research Dosage Ranges

ProtocolDoseFrequency
Standard fat loss research300 mcg/dayDaily, fasted
Enhanced protocol500 mcg/dayDaily, fasted
Higher dose trials1000 mcg/dayDivided doses

The most commonly referenced dose from clinical research is 300–500 mcg/day, administered subcutaneously in a fasted state.

Injection Volume Table

Using a 5000 mcg/mL solution (5 mg in 1 mL BW):

Target DoseVolume to Inject
200 mcg0.04 mL (4 units)
300 mcg0.06 mL (6 units)
500 mcg0.10 mL (10 units)
1000 mcg0.20 mL (20 units)

Using a 2500 mcg/mL solution (5 mg in 2 mL BW):

Target DoseVolume to Inject
300 mcg0.12 mL (12 units)
500 mcg0.20 mL (20 units)
1000 mcg0.40 mL (40 units)

Use the Dose Calculator to calculate exact volumes for any concentration.

Frequency, Timing, and Cycle Length

  • Timing: Fasted state is critical — administer 30–60 minutes before first meal (morning injection on an empty stomach)
  • Frequency: Once daily
  • Cycle length: 8–12 weeks; some research extends to 20 weeks
  • Injection site: Subcutaneous to abdomen is most common; closer to fat deposits that are targeted

Storage

  • Lyophilised vials: -20°C for long-term; 2–8°C short-term
  • After reconstitution: 2–8°C; use within 28 days

Calculate Your Exact Injection Volume

Use our free dose calculator — enter any vial size and target dose to get the precise volume to draw up.

About the Author

KR

KnowYourPeptide Research Team

KnowYourPeptide Research Team

Content produced by the KnowYourPeptide research and editorial team. All articles are written from peer-reviewed primary literature and reviewed for scientific accuracy by credentialed researchers and a board-certified physician before publication.

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Medically Reviewed by Dr. Amanda Reid, MD

This article has been reviewed by Dr. Amanda Reid, MD (Board-Certified Internal Medicine), Know Your Peptide Medical Advisor, for scientific accuracy, safety information, and appropriate clinical context. Learn about our review process.

Research Profiles Referenced in This Article