BPC-157 Dosage Guide: Reconstitution, Dosing Tables, and Injection Protocol

BPC-157 (Body Protection Compound-157) is one of the most frequently researched healing peptides, with over 150 published preclinical studies covering gastrointestinal, tendon, ligament, muscle, and neural repair. This guide covers vial reconstitution, injection volume calculation, and standard research dosing protocols.

What Vials Typically Come In

BPC-157 research vials are commonly available in:

• 5 mg vials (most standard)
• 10 mg vials (for higher-frequency protocols)
• 2 mg vials (occasionally available)

Always verify with your certificate of analysis.

How to Reconstitute BPC-157

BPC-157 is reconstituted with bacteriostatic water. The peptide is highly water-soluble and dissolves readily.

Standard reconstitution for a 5 mg vial:

1. Clean the rubber stopper with an alcohol swab

2. Draw 1–2 mL of bacteriostatic water into a syringe

3. Inject water slowly down the vial wall (avoid directing at the powder)

4. Swirl very gently — do not shake or vortex

5. Allow 1–2 minutes for complete dissolution

6. Solution should be clear; discard if particulate matter is present

Concentration examples for a 5 mg vial:

• Add 1 mL BW → 5000 mcg/mL
• Add 2 mL BW → 2500 mcg/mL
• Add 5 mL BW → 1000 mcg/mL

The 2 mL (2500 mcg/mL) dilution is a popular working concentration as it provides convenient volumes for the 250–500 mcg dose range.

Standard Research Dosage Ranges

Most published rodent studies use weight-based dosing of 2–10 mcg/kg. The most commonly referenced human research extrapolation is 250–500 mcg per injection.

Injection Volume Table

Using a 2500 mcg/mL reconstituted solution (5 mg in 2 mL BW):

Use the Dose Calculator to instantly convert any dose and vial size to injection volume.

Frequency and Timing

• Frequency: Once daily is the most common protocol; some studies use twice daily for accelerated healing
• Timing: No strict timing requirement; consistent daily administration is more important than time of day
• Injection site: Subcutaneous injection near the injury site is preferred in some models, though systemic subcutaneous has been shown effective as well
• Oral: BPC-157 is also effective orally for gastrointestinal applications; the same dose range applies

Cycle Length

• Acute injury: 4–8 weeks
• Chronic conditions / GI research: 8–12 weeks
• Extended research: Protocols up to 6 months have been used without significant safety findings in animal models

Storage

• Lyophilised powder: -20°C until needed
• After reconstitution: 2–8°C refrigerator
• Use within 28 days of reconstitution
• Protect from light; avoid freeze-thaw cycles after reconstitution