Muscle & Performance

ACE-031

A soluble activin type IIB receptor fusion protein that sequesters myostatin and other TGF-beta family members to drive skeletal muscle hypertrophy and fat loss.

C₃₄₁₈H₅₁₈₈N₉₂₈O₁₀₆₂S₃₈Half-life: 10-15 daysMolar mass: 77489.82 g/mol

⚠ Research & Educational Use Only. ACE-031 is a research chemical documented here for scientific education. All information references peer-reviewed literature and preclinical/clinical study data. Not for human consumption. Not medical advice. Consult a licensed researcher or healthcare professional before any laboratory use.

Medically reviewed by Dr. Amanda Reid, MDWritten by the KnowYourPeptide Research TeamLast updated April 2026
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Key Takeaways
  • Sequesters myostatin - the primary negative regulator of muscle growth - preventing it from binding ActRIIB in muscle
  • Also inhibits activins and other GDF ligands that suppress muscle growth through the same receptor
  • Demonstrated significant lean mass increases in clinical Phase 1 research - one of the most potent experimental muscle agents studied
  • ACE-031 is not FDA-approved for human use. It is a research chemical for scientific study only.

Research At a Glance

  • Sequesters myostatin - the primary negative regulator of muscle growth - preventing it from binding ActRIIB in muscle
  • Also inhibits activins and other GDF ligands that suppress muscle growth through the same receptor
  • Demonstrated significant lean mass increases in clinical Phase 1 research - one of the most potent experimental muscle agents studied
  • Reduces fat mass simultaneously in research subjects - likely via activin pathway effects on adipose tissue
Calculate ACE-031 dose

What is ACE-031?

ACE-031 (soluble activin receptor type IIB, or ActRIIB-IgG1-Fc) is a fusion protein consisting of the extracellular domain of the activin receptor type IIB (ActRIIB) fused to an immunoglobulin Fc region. It functions as a "ligand trap" - circulating in the bloodstream and binding to myostatin, activins, and other TGF-beta superfamily members that would otherwise suppress muscle growth.

Myostatin (GDF-8) is a secreted growth factor that acts as the primary brake on skeletal muscle mass. Animals with genetic myostatin deficiency (or mutations in the myostatin pathway) exhibit extraordinary muscular development - the "double-muscled" phenotype observed in Belgian Blue cattle, Piedmontese cattle, and rare human cases of myostatin-null mutations. Blocking myostatin therefore represents one of the most direct routes to skeletal muscle hypertrophy.

However, ACE-031's mechanism goes beyond simple myostatin inhibition. Because it presents the actual receptor binding domain, it traps any molecule that naturally binds ActRIIB - including multiple GDF (growth differentiation factor) family members and activins. This broader inhibition is a double-edged sword: it may produce more potent muscle growth than selective myostatin antibodies, but it also affects vascular biology, since ActRIIB is expressed in vascular endothelial cells.

ACE-031 was developed by Acceleron Pharma and advanced to Phase 2 clinical trials for Duchenne muscular dystrophy. The Phase 1 results were remarkable - even single doses produced significant increases in thigh muscle volume and reductions in fat mass. However, Phase 2 was halted when an unacceptable rate of bleeding events (nosebleeds, gum bleeding) and telangiectasia were observed, attributed to ActRIIB's role in vascular endothelial cell biology.

This safety finding distinguishes ACE-031 from more selective myostatin approaches and explains why the clinical program was discontinued. Research continues into whether selective myostatin blockade (without broader ActRIIB inhibition) can achieve similar muscle benefits without vascular effects.

Key Research Benefits

Documented effects observed in preclinical and clinical studies on ACE-031. See all Muscle & Performance peptides for comparison.

Sequesters myostatin - the primary negative regulator of muscle growth - preventing it from binding ActRIIB in muscle
Also inhibits activins and other GDF ligands that suppress muscle growth through the same receptor
Demonstrated significant lean mass increases in clinical Phase 1 research - one of the most potent experimental muscle agents studied
Reduces fat mass simultaneously in research subjects - likely via activin pathway effects on adipose tissue
Long half-life (10-15 days) allows infrequent dosing
Improves bone density in models of muscular dystrophy - ActRIIB signalling regulates bone as well as muscle
Research interest in Duchenne muscular dystrophy, cachexia, and muscle wasting conditions
Mechanism is independent of androgen or GH pathways - additive with anabolic hormones

Common Stacks

ACE-031 is frequently combined with the following peptides for synergistic effects. Click any peptide to compare profiles before deciding.

CJC-1295 drives endogenous IGF-1 production via GH; combining with exogenous IGF-1 LR3 amplifies the anabolic signal significantly.

CJC-1295 profile

Ipamorelin + CJC-1295 as a GH stack pairs with IGF-1 LR3 for a comprehensive GH-axis and direct IGF-1 receptor anabolic protocol.

Ipamorelin profile

IGF-1 LR3 provides systemic long-acting IGF-1 exposure while IGF-1 DES can be used for localised site-specific anabolism.

IGF-1 DES profile

Side Effects & Risks

Adverse effects reported in the research literature. All data sourced from preclinical and clinical study reports.

Epistaxis (nosebleeds) and gum bleeding - likely related to ActRIIB expression in vascular endothelium; caused Phase 2 trial discontinuation
Telangiectasia (small vascular dilations) reported in Phase 1 human trials
Spider veins and vascular skin changes
Potential for excessive muscle hypertrophy if dosing is not carefully controlled
Limited long-term safety data - clinical program was suspended
Dizziness and headache reported in early trials

Dosing Data from the Literature

Doses referenced below are sourced from published preclinical and clinical studies. Use the peptide dose calculator to convert these values to injection volume.

Research Dosing Protocol

ACE-031's Phase 1 clinical research used single doses of 1-3 mg/kg subcutaneously. Given its 10-15 day half-life, dosing frequency is very low.

Clinical trial doses (reference only): 0.03-3 mg/kg single dose SC Half-life: 10-15 days - monthly or bi-monthly dosing in sustained protocols NOTE: The Phase 2 clinical program was suspended due to vascular adverse events (bleeding, telangiectasia). Research use should carefully monitor for these effects.

Enter your vial size and target dose to get the exact injection volume.

Administration in Research Settings

Standard reconstitution and administration methodology for laboratory research use.

ACE-031 is a large protein (IgG1-Fc fusion) requiring subcutaneous injection. Reconstitute with sterile water for injection. Administer once monthly or less frequently given the extended half-life.

Explore Further

Quick Reference

Half-Life
10-15 days
Molar Mass
77489.82 g/mol
Formula
C₃₄₁₈H₅₁₈₈N₉₂₈O₁₀₆₂S₃₈
Legal Status
Research chemical/experimental biologic. Clinical trials discontinued. Not approved for any use.
Storage
Refrigerate at 2-8 degrees C. Avoid freezing. Reconstituted solution: use within 24-48 hours.

Research Use Only

This information is for educational research purposes only. This is not medical advice. Consult a qualified healthcare professional.